Figure 6
mismatches placed at the margins of oligonucleotides are hard to detect. A) 3D histogram grouping oligonucleotides according to mismatch location and signal ratio (log2 scaled). Only oligonucleotides covering a single nucleotide polymorphism (SNP) with the corresponding PA14 sequence are included. Each rectangle represents the group of all oligonucleotides with a SNP at the specified position (x-axis) showing the specified hybridization ratio (y-axis). The dashed yellow line indicates the average hybridization ratio of all oligonucleotides with common mismatch location. Error bars indicate one standard deviation. B) Sensitivity (see Figure 5) for detection of oligonucleotides covering a SNP (D th = -0.5).