Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome

Table 4 Signal ratios of SNPs are biased towards the interaction type (strong or weak) of the bases present in the probe and sample alleles.

PAO1 allele (probe)^a	PA14 allele (sample)^a	abundance	median signal ratio	sensitivity^b
W	S	15364	-0.53	52.1%
W	W	657	-0.46	47.5%
S	S	2592	-0.38	43.7%
S	W	12972	-0.30	39.1%
Σ		31585	-0.43	45.9%

^a W – IUPAC format for bases with weak interaction (A, T), S – strong interaction (G, C).
^b determined for D_th= -0.5.

ISSN: 1471-2164