(A) Hierarchical clustering analysis using the 146 transcripts that did not correlate with karyotype. Genes of the two sex chromosome gene clusters (see D and E in Figure 1) were excluded from calculating the dendrograms, but are displayed in the figure to show their distribution across the samples. All color codes are the same as in figure 1. (B) Enlarged dendrogram demonstrating that major subdivision of the individuals mirrors the degree of genital virilization independent of karyotype. (C). Mean Prader stage of genital virilization (± 1 SD) of the DSD individuals the main branches of the dendrogram excluding the normal controls. The mean Prader stage on the left side is Prader "1.6" and "3.2" on the right (p = 0.02) [see also additional files 6, 7, 8, 9].