BAC contigs established by chromosome walking. BAC clones are represented as black and grey horizontal lines and BES used as probes are represented as black (SP6-end) and grey (T7-end) circles. The physical positions of BAC clones represented by grey lines were determined in FISH experiments. The number and library origin of each BAC clone is shown where M indicates the Mde library, and H indicates the Hf library. The FPC-based contig of each BAC is shown in parenthesis (NF indicates a clone that was not fingerprinted). Black vertical arrows intersect BAC clones that were positive in library screens that used STS markers as probe. Grey vertical lines intersect BAC clones that were positive in library screens that used BES as probe. (a) Chromosome walk-124. (b) Chromosome walk-134.