Hessian fly polytene chromosomes and BAC clone co-localizations. Chromosome segments (A-Z) are indicated on geimsa stained chromosomes (a, f, k, and p). White bars (10 μm) indicate the relative lengths of corresponding DAPI stained chromosomes (b, g, l, and q). Four examples of co-localizing BAC clones are shown in color overlay images (e, j, o, and t). Contig #320 BAC clones CL4h4 (c) and CL31e21 (d) hybridized to chromosome A1 segment G (e). Contig #199 BAC clones Hf30g15 (h) and Hf4e2 (i) hybridized to chromosome A2 segment L (j). Note that Hf30g15 hybridization (green) was more distal than Hf4e2 hybridization (red), allowing contig orientation. Contig #320 BAC clones CL25e22 (m) and Hf10j14 (n) hybridized to chromosome X1 segment U (o). Contig #320 BAC clones CL29m24 (r) and Hf10j14 (s) hybridized to chromosome X2 segment X (t).