Figure 3
From: A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes
Resolving "invalid" contigs using FISH. (a) The hybridization of BACs CL24B4 (green arrow, A2 segment L) and Hf15M12 (red arrows, pericentromeric heterochromatin) defined contig #36 as invalid and repetitive. (b) The hybridization of a third contig #36 BAC (CL24N4, yellow arrow) resolved the position of contig #36 to A2 segment L. (c) The hybridization of BACs CL17J4 (green arrow, A2 segment L) and Hf7B18 (red arrows, A1 segment J) defined contig #234 as invalid but non-repetitive. (d) Hybridization of a third contig #234 BAC clone (Hf13K2, yellow arrow) with BAC clone CL17J4 resolved the position of contig #234 to A2 segment L.