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Table 3 Assessment of 196 contigs using two BAC clones per contig as FISH probes.

From: A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes

  Valid Invalid Invalid-R Invalid-NR
Total no. contigs 169 27 11 16
Total no. BACs 3208 575 186 389
Mean no. BACs/contig 19 ± 10 21 ± 11 17 ± 11 24 ± 10*†
Total no. Qs 641 161 41 120
Total Qs/BAC 0.20 0.28 0.22 0.31
Mean no. Qs/contig 3.8 ± 4.7 6.0 ± 5.3* 3.7 ± 5.7 7.5 ± 4.6**†
Mean no. Qs/BAC/contig 0.16 ± 0.13 0.25 ± 0.17** 0.15 ± 0.16 0.31 ± 0.15**††
Total CB units
(estimated kb)
58,255
(68,857)
10,214
(12,072)
3,563
(4,211)
6,651
(7,861)
Mean CB units/contig
(estimated kb/contig)
344 ± 129
(407 ± 152)
378 ± 148
(447 ± 175)
324 ± 126
(383 ± 150)
415 ± 154*
(491 ± 182)
  1. Contigs in which the probes co-localized were assessed as valid. Contigs in which the probes failed to co-localize were assessed as invalid. Invalid contigs were further divided into contigs that either had one or more repetitive BAC probes (Invalid-R) or no repetitive BAC probes (Invalid-NR). *Means significantly different from the Valid mean (Student's t; p < 0.10). **Means significantly different from the Valid mean (Student's t; p < 0.05). Means significantly different from the Invalid-R mean (Student's t; p < 0.10). ††Means significantly different from the Invalid-R mean (Student's t; p < 0.05).