A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes

BMC Genomics

Table 3 Assessment of 196 contigs using two BAC clones per contig as FISH probes.

	Valid	Invalid	Invalid-R	Invalid-NR
Total no. contigs	169	27	11	16
Total no. BACs	3208	575	186	389
Mean no. BACs/contig	19 ± 10	21 ± 11	17 ± 11	24 ± 10^*†
Total no. Qs	641	161	41	120
Total Qs/BAC	0.20	0.28	0.22	0.31
Mean no. Qs/contig	3.8 ± 4.7	6.0 ± 5.3*	3.7 ± 5.7	7.5 ± 4.6^**†
Mean no. Qs/BAC/contig	0.16 ± 0.13	0.25 ± 0.17**	0.15 ± 0.16	0.31 ± 0.15^**††
Total CB units (estimated kb)	58,255 (68,857)	10,214 (12,072)	3,563 (4,211)	6,651 (7,861)
Mean CB units/contig (estimated kb/contig)	344 ± 129 (407 ± 152)	378 ± 148 (447 ± 175)	324 ± 126 (383 ± 150)	415 ± 154* (491 ± 182)

Contigs in which the probes co-localized were assessed as valid. Contigs in which the probes failed to co-localize were assessed as invalid. Invalid contigs were further divided into contigs that either had one or more repetitive BAC probes (Invalid-R) or no repetitive BAC probes (Invalid-NR). *Means significantly different from the Valid mean (Student's t; p < 0.10). **Means significantly different from the Valid mean (Student's t; p < 0.05). ^†Means significantly different from the Invalid-R mean (Student's t; p < 0.10). ^††Means significantly different from the Invalid-R mean (Student's t; p < 0.05).

ISSN: 1471-2164