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Table 2 Summary of the features of two-dimensional experiment

From: Differential expression proteomics to investigate responses and resistance to Orobanche crenata in Medicago truncatula

Feature Staining
  Coomassie Silver
Protein/gel 500 μg 100 μg
Total spots resolved 400 800
Spot showing significant differences:   
- Totala 60 (15%) 76 (9.5%)
- Between genotypes 25 44
- Between control vs. inoculated SA4087 plants 20 22
- Between control vs. inoculated SA27774 plants 15 10
Total identified spots 35 14
Valid identified spotsb 26 14
Unique proteinsc 26 13
  1. a 31 differential spots were observed with both staining methods
  2. b Spots identified with reasonable values for score, % coverage, number of matched peptides, and observed Mr and pI values close to theoretical data.
  3. c Number of different proteins identified.