Differential expression proteomics to investigate responses and resistance to Orobanche crenata in Medicago truncatula

Table 2 Summary of the features of two-dimensional experiment

Feature	Staining
	Coomassie	Silver
Protein/gel	500 μg	100 μg
Total spots resolved	400	800
Spot showing significant differences:
- Total^a	60 (15%)	76 (9.5%)
- Between genotypes	25	44
- Between control vs. inoculated SA4087 plants	20	22
- Between control vs. inoculated SA27774 plants	15	10
Total identified spots	35	14
Valid identified spots^b	26	14
Unique proteins^c	26	13

^a 31 differential spots were observed with both staining methods
^b Spots identified with reasonable values for score, % coverage, number of matched peptides, and observed Mr and pI values close to theoretical data.
^c Number of different proteins identified.

ISSN: 1471-2164