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Table 1 Summary of LongSAGE tags sequenced from B. napus seeds at 23 and 35 days after pollination (DAP).

From: Gene expression profiling via LongSAGE in a non-model plant species: a case study in seeds of Brassica napus

  23 DAP library 35 DAP library Combined data
Category Unique sequences All sequences Unique sequences All sequences Unique sequences All sequences
Total extracted ditags - 34,984 - 15,919 - 50,903
Rejected ditags (Phred score ≤ 20a) - 10,685 - 3,306 - 13,991
Accepted ditags (Phred score > 20a) 23,996 24,299 11,846 12,613 35,842 36,912
Total extracted tags 24,655 48,534 12,714 25,198 37,369 73,732
Accepted tags (excluding polyA tags) 24,654 48,514 12,713 25,183 37,367 73,697
Copy number of accepted tags
1 18,183 (73.8.%) 18,183 (37.5%) 9,741 (76.6%) 9,741 (38.7%)   
2–5 5,329 (21.6%) 14,320 (29.5%) 2,510 (19.7%) 6,596 (26.2%)   
6–20 1,016 (4.1%) 9,450 (19.5%) 406 (3.2%) 3,668 (14.6%)   
21–99 118 (0.5%) 4,437 (9.1%) 46 (0.4%) 1,997 (7.9%)   
>100 8 (0.03%) 2,124 (4.4%) 9 (0.07%) 3,181 (12.6%)   
  1. a Using Sage-phred.pl, including duplicated ditags