Table 1 Summary of LongSAGE tags sequenced from B. napus seeds at 23 and 35 days after pollination (DAP).
| Â | 23 DAP library | 35 DAP library | Combined data | |||
|---|---|---|---|---|---|---|
Category | Unique sequences | All sequences | Unique sequences | All sequences | Unique sequences | All sequences |
Total extracted ditags | - | 34,984 | - | 15,919 | - | 50,903 |
Rejected ditags (Phred score ≤ 20a) | - | 10,685 | - | 3,306 | - | 13,991 |
Accepted ditags (Phred score > 20a) | 23,996 | 24,299 | 11,846 | 12,613 | 35,842 | 36,912 |
Total extracted tags | 24,655 | 48,534 | 12,714 | 25,198 | 37,369 | 73,732 |
Accepted tags (excluding polyA tags) | 24,654 | 48,514 | 12,713 | 25,183 | 37,367 | 73,697 |
Copy number of accepted tags | ||||||
1 | 18,183 (73.8.%) | 18,183 (37.5%) | 9,741 (76.6%) | 9,741 (38.7%) | Â | Â |
2–5 | 5,329 (21.6%) | 14,320 (29.5%) | 2,510 (19.7%) | 6,596 (26.2%) |  |  |
6–20 | 1,016 (4.1%) | 9,450 (19.5%) | 406 (3.2%) | 3,668 (14.6%) |  |  |
21–99 | 118 (0.5%) | 4,437 (9.1%) | 46 (0.4%) | 1,997 (7.9%) |  |  |
>100 | 8 (0.03%) | 2,124 (4.4%) | 9 (0.07%) | 3,181 (12.6%) | Â | Â |