Table 2 Summary of LongSAGE tag-to-gene mapping data for two libraries from B. napus seeds at 23 and 35 days after pollination (DAP).
Analysis including all singleton tags | 23 DAP library | 35 DAP library | combined data |
|---|---|---|---|
Number of Brassica ESTs matched to the A. thaliana proteome | 834,732 | ||
Number of A. thaliana genes matched by Brassica ESTs | 20,462 | ||
Number of tags accepted for processing | 24,654 | 12,713 | 32,395 |
Number of Brassica ESTs matched by tags | 256,420 | 214,981 | 301,180 |
Total number of accepted tags matched to Brassica ESTs | 12,728 of 24,654 (51.6%) | 7,232 of 12,713 (56.9%) | 15,955 of 32,395 (49.3%) |
Number of accepted tags not matched to any Brassica EST | 11,926 of 24,654 (48.4%) | 5,481 of 12,713 (43.1%) | 16,400 of 32,395 (50.6%) |
Number of accepted singleton tags matched to Brassica ESTs | 7,615 of 18,183 (41.9%) | 4,819 of 9,741 (49.5%) | 11,421 of 26,498 (43.1%) |
Number of accepted tags with ≥2 counts matched to Brassica ESTs | 5,513 of 6,471 (85.2%) | 2,413 of 2,972 (81.2%) | 6,010 of 7,727 (77.8%) |
Number of A. thaliana genes matched via Brassica ESTs and tags | 7,208 | 4,875 | 8,255 |
Number of A. thaliana genes matched and oriented via Brassica ESTs and tags (putative chimeric ESTs removed) | 4,478 | 3,005 | 5,120 |
Number of genes matched in both directions | 1,125 (25.1%) | 560 (18.6%) | 1,453 (28.4%) |
Number of genes matched in sense orientation exclusively | 3,057 (68.3%) | 2,242 (74.6%) | 3,358 (69.5%) |
Number of genes matched in antisense orientation exclusively | 296 (6.6%) | 203 (6.8%) | 309 (6.1%) |