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Table 2 Summary of LongSAGE tag-to-gene mapping data for two libraries from B. napus seeds at 23 and 35 days after pollination (DAP).

From: Gene expression profiling via LongSAGE in a non-model plant species: a case study in seeds of Brassica napus

Analysis including all singleton tags 23 DAP library 35 DAP library combined data
Number of Brassica ESTs matched to the A. thaliana proteome 834,732
Number of A. thaliana genes matched by Brassica ESTs 20,462
Number of tags accepted for processing 24,654 12,713 32,395
Number of Brassica ESTs matched by tags 256,420 214,981 301,180
Total number of accepted tags matched to Brassica ESTs 12,728 of 24,654 (51.6%) 7,232 of 12,713 (56.9%) 15,955 of 32,395 (49.3%)
Number of accepted tags not matched to any Brassica EST 11,926 of 24,654 (48.4%) 5,481 of 12,713 (43.1%) 16,400 of 32,395 (50.6%)
Number of accepted singleton tags matched to Brassica ESTs 7,615 of 18,183 (41.9%) 4,819 of 9,741 (49.5%) 11,421 of 26,498 (43.1%)
Number of accepted tags with ≥2 counts matched to Brassica ESTs 5,513 of 6,471 (85.2%) 2,413 of 2,972 (81.2%) 6,010 of 7,727 (77.8%)
Number of A. thaliana genes matched via Brassica ESTs and tags 7,208 4,875 8,255
Number of A. thaliana genes matched and oriented via Brassica ESTs and tags (putative chimeric ESTs removed) 4,478 3,005 5,120
Number of genes matched in both directions 1,125 (25.1%) 560 (18.6%) 1,453 (28.4%)
Number of genes matched in sense orientation exclusively 3,057 (68.3%) 2,242 (74.6%) 3,358 (69.5%)
Number of genes matched in antisense orientation exclusively 296 (6.6%) 203 (6.8%) 309 (6.1%)