Table 4 Summary of the 20 most abundant genes expressed in B. napus seeds at 23 days after pollination (DAP) based on LongSAGE data analysis.
Rank | A. thaliana locus | No. of matched Brassica ESTs at 1e-6 | No. of different tags matched to Brassica ESTs | Mean tag count per locus | Frequency of tag counts (%, normalized) | Description of matched A. thaliana gene locus |
|---|---|---|---|---|---|---|
1 | AT5G45890 | 4,452 | 44 | 772 | 1.590 | SAG12 (SENESCENCE-ASSOCIATED GENE 12); cysteine-type peptidase |
2 | AT5G50260 | 109 | 15 | 320 | 0.660 | cysteine proteinase, putative |
3 | AT3G48350 | 14 | 7 | 299 | 0.615 | cysteine proteinase, putative |
4 | AT5G38195 | 1,640 | 36 | 263 | 0.541 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
5 | AT1G48750 | 8 | 4 | 209 | 0.430 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
6 | AT3G20210 | 630 | 13 | 156 | 0.323 | DELTA-VPE (delta vacuolar processing enzyme); cysteine-type endopeptidasee |
7 | AT2G12465 | 160 | 11 | 134 | 0.275 | LCR50 (Low-molecular-weight cysteine-rich 50) |
8 | AT4G32110 | 852 | 20 | 127 | 0.263 | transferase, transferring glycosyl groups |
9 | AT3G04120 | 814 | 30 | 124 | 0.256 | GAPC (GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C SUBUNIT) |
10 | AT5G37474 | 35 | 10 | 120 | 0.247 | Encodes a defensin-like (DEFL) family protein |
11 | AT2G14846 | 191 | 13 | 118 | 0.244 | protease inhibitor/seed storage/lipid transfer protein (LTP) family proteinperoxidise |
12 | AT4G21960 | 1,312 | 16 | 118 | 0.243 | PRXR1 (peroxidase 42); peroxidase |
13 | AT5G60390 | 2,771 | 30 | 113 | 0.233 | elongation factor 1-alpha/EF-1-alpha |
14 | AT4G32105 | 79 | 13 | 109 | 0.225 | Galactosyltransferase |
15 | AT1G58055 | 23 | 5 | 100 | 0.206 | Encodes a defensin-like (DEFL) family protein |
16 | AT1G04645 | 502 | 11 | 97 | 0.200 | self-incompatibility protein-related |
17 | AT1G05850 | 426 | 11 | 96 | 0.198 | POM1; chitinase |
18 | AT1G08830 | 410 | 14 | 95 | 0.196 | CSD1 (copper/zinc superoxide dismutase 1) |
19 | AT4G12960 | 351 | 6 | 93 | 0.191 | gamma interferon responsive lysosomal thiol reductase family protein/GILT |
20 | AT4G21650 | 53 | 8 | 92 | 0.189 | subtilase family protein |