Table 5 Summary of the 20 most abundant genes expressed in B. napus seeds at 35 days after pollination (DAP) based on LongSAGE data analysis.
Rank | A. thaliana locus | No. of matched Brassica ESTs at 1e-6 | No. of different tags matched to Brassica ESTs | Mean tag count per locus | Frequency of tag counts (%, normalized) | Description of matched A. thaliana gene locus |
|---|---|---|---|---|---|---|
1 | AT4G27140 | 16,413 | 96 | 669 | 2.657 | 2S seed storage protein 1/2S albumin storage protein/NWMU1-2S albumin 1 |
2 | AT4G27170 | 6,691 | 67 | 637 | 2.528 | 2S seed storage protein 4/2S albumin storage protein/NWMU2-2S albumin 4 |
3 | AT4G27160 | 2,402 | 34 | 575 | 2.284 | 2S seed storage protein 3/2S albumin storage protein/NWMU2-2S albumin 3 |
4 | AT4G27150 | 249 | 24 | 560 | 2.224 | 2S seed storage protein 2/2S albumin storage protein/NWMU2-2S albumin 2 |
5 | AT4G28520 | 126 | 13 | 195 | 0.776 | CRU3 (CRUCIFERIN 3) |
6 | AT3G27660 | 1,226 | 15 | 183 | 0.728 | OLEO4 (OLEOSIN4) |
7 | AT5G38195 | 1,640 | 36 | 161 | 0.640 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
8 | AT4G25140 | 1,119 | 15 | 146 | 0.580 | OLEO1 (OLEOSIN1) |
9 | AT5G44120 | 1,112 | 17 | 143 | 0.567 | CRA1 (CRUCIFERINA); nutrient reservoir |
10 | AT5G45890 | 4,452 | 44 | 143 | 0.566 | SAG12 (SENESCENCE-ASSOCIATED GENE 12); cysteine-type peptidase |
11 | AT1G03880 | 1,791 | 12 | 138 | 0.549 | CRU2 (CRUCIFERIN 2); nutrient reservoir |
12 | AT1G48750 | 8 | 4 | 136 | 0.538 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
13 | AT1G79870 | 5 | 3 | 123 | 0.487 | oxidoreductase family protein |
14 | AT1G12920 | 1 | 2 | 113 | 0.447 | ERF1-2 (EUKARYOTIC RELEASE FACTOR 1-2); translation release factor |
15 | AT5G35530 | 401 | 6 | 102 | 0.406 | 40S ribosomal protein S3 (RPS3C) |
16 | AT3G12580 | 31 | 8 | 102 | 0.405 | HSP70 (heat shock protein 70); ATP binding |
17 | AT3G01570 | 170 | 6 | 99 | 0.393 | glycine-rich protein/oleosin |
18 | AT1G69830 | 1 | 2 | 97 | 0.385 | AMY3/ATAMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase |
19 | AT3G05020 | 401 | 14 | 86 | 0.340 | ACP1 (ACYL CARRIER PROTEIN 1) |
20 | AT5G09440 | 31 | 3 | 82 | 0.326 | phosphate-responsive protein, putative |