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Table 5 Summary of the 20 most abundant genes expressed in B. napus seeds at 35 days after pollination (DAP) based on LongSAGE data analysis.

From: Gene expression profiling via LongSAGE in a non-model plant species: a case study in seeds of Brassica napus

Rank A. thaliana locus No. of matched Brassica ESTs at 1e-6 No. of different tags matched to Brassica ESTs Mean tag count per locus Frequency of tag counts (%, normalized) Description of matched A. thaliana gene locus
1 AT4G27140 16,413 96 669 2.657 2S seed storage protein 1/2S albumin storage protein/NWMU1-2S albumin 1
2 AT4G27170 6,691 67 637 2.528 2S seed storage protein 4/2S albumin storage protein/NWMU2-2S albumin 4
3 AT4G27160 2,402 34 575 2.284 2S seed storage protein 3/2S albumin storage protein/NWMU2-2S albumin 3
4 AT4G27150 249 24 560 2.224 2S seed storage protein 2/2S albumin storage protein/NWMU2-2S albumin 2
5 AT4G28520 126 13 195 0.776 CRU3 (CRUCIFERIN 3)
6 AT3G27660 1,226 15 183 0.728 OLEO4 (OLEOSIN4)
7 AT5G38195 1,640 36 161 0.640 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein
8 AT4G25140 1,119 15 146 0.580 OLEO1 (OLEOSIN1)
9 AT5G44120 1,112 17 143 0.567 CRA1 (CRUCIFERINA); nutrient reservoir
10 AT5G45890 4,452 44 143 0.566 SAG12 (SENESCENCE-ASSOCIATED GENE 12); cysteine-type peptidase
11 AT1G03880 1,791 12 138 0.549 CRU2 (CRUCIFERIN 2); nutrient reservoir
12 AT1G48750 8 4 136 0.538 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein
13 AT1G79870 5 3 123 0.487 oxidoreductase family protein
14 AT1G12920 1 2 113 0.447 ERF1-2 (EUKARYOTIC RELEASE FACTOR 1-2); translation release factor
15 AT5G35530 401 6 102 0.406 40S ribosomal protein S3 (RPS3C)
16 AT3G12580 31 8 102 0.405 HSP70 (heat shock protein 70); ATP binding
17 AT3G01570 170 6 99 0.393 glycine-rich protein/oleosin
18 AT1G69830 1 2 97 0.385 AMY3/ATAMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase
19 AT3G05020 401 14 86 0.340 ACP1 (ACYL CARRIER PROTEIN 1)
20 AT5G09440 31 3 82 0.326 phosphate-responsive protein, putative