Figure 3

Immunohistochemistry demonstrating inflammatory cells in the suprarenal aorta of mice with aneurysms demonstrating functional confirmation of microarray expression data. Boxplots show normalised expression of genes that are markers of various haematopoietic cell lineages; Csf3r (neutrophils), Cd3d (T lymphocytes), Blnk (B lymphocytes), Itgax (dendritic cells) and Cd68 (monocyte/macrophages) from aneurysmal (n = 5), non-aneurysmal (n = 7) and saline control (n = 6) mice. Shown are sections stained for (A) neutrophils, (B) T lymphocytes (C) B lymphocytes (D) dendritic cells (E), macrophages in mice with aneurysms (i) and those without aortic dilatation (ii). Scale bars represent 100 μm.