Table 1 Statistics of the high quality reads from four GS20–454 sequencing runs of the Palomero cDNA library and coverage of the maize unigenes from the NCBI (UniGene) and TIGR (ZMGI) databases after each sequencing run.
From: Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing
Run GS20–454 | Num. reads (Mb) | Avg length of read (nt) | Cumulative runs | ZMGI (N = 115744 seq) | NCBI (N = 55327 seq) | ||
|---|---|---|---|---|---|---|---|
Matches (%) | Increase (%) | Matches (%) | Increase (%) | ||||
1 | 367391 (37.09) | 100.94 | 1 | 36.42 | 0 | 37.89 | 0 |
2 | 367699 (37.04) | 100.73 | + 2 | 43.58 | 7.16 | 44.12 | 6.23 |
3 | 394851 (39.73) | 100.62 | + 3 | 47.83 | 4.25 | 47.74 | 3.62 |
4 | 387937 (38.99) | 100.50 | + 4 | 50.42 | 2.59 | 50.00 | 2.26 |
Total | 1517878 (152.37) | 100.38 | |||||