Skip to main content

Table 6 Comparison of transcript abundance of representative maize genes estimated by GS20–454 sequencing and qPCR.

From: Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing

Gene locus matched Accession (ZMGI) Ra454 reads Ct qPCR (mean ± SD)
Similar to UP|LIRP1_ORYSA (Q03200) Light-regulated protein precursor TC327885 2.11 11.7808 ± 0.09
Similar to UP|PSAE_HORVU (P13194) Photosystem I reaction center subunit IV, chloroplast precursor (PSI-E) TC361477 0.74 13.1666 ± 0.05
UP|Q41754_MAIZE (Q41754) Ubiquitin TC369342 0.49 14.6629 ± 0.10
UP|TBA6_MAIZE (P33627) Tubulin alpha-6 chain (Alpha-6 tubulin) TC326717 0.32 14.0284 ± 0.03
Homologue to GB|BAD33626.1|50726105|AP005579 Polyubiquitin 2 TC342043 0.32 16.3357 ± 0.04
Homologue to UP|Q41772_MAIZE (Q41772) Cytosolic ascorbate peroxidase, complete TC364641 0.22 16.5425 ± 0.08
Similar to UP|SODC2_MESCR (O49044) Superoxide dismutase [Cu-Zn]2 TC327155 0.08 17.9920 ± 0.08
Similar to UP|Q6YIH2_ORYSA (Q6YIH2) OsCDPK protein TC327230 0.06 18.5091 ± 0.13