Phenotypic anchoring measurements for male fathead minnows. (A and B) Experiment 1. (A) Plasma T concentrations (ng/mL), (B) Plasma vitellogenin concentrations (μg/mL) in the same fish samples. (Cntrl) triethylene glycol control, (EE2-10) 10 ng EE2/L, (EE2-50) 50 ng EE2/L, (ZM-100) 100 ng ZM/L, (Mix 1:2) 50 ng EE2/L and 100 ng ZM/L. (C) Experiment 2. Plasma VTG concentrations (μg/mL). (EE2-5) 5 ng EE2/L; (Mix 1:20) 5 ng EE2/L and 100 ng ZM/L. Significance ** P ≤ 0.001 and * P ≤ 0.05.