Histograms to evaluate the models. Genes isolated from S. purpuratus (circles), vertebrates (diamonds), and protostomes (triangles) were collected and analyzed using the Purpuratus (A) and Vertebrate (B) models. Histograms of the known positive (solid lines) and negative (dashed lines) donor splice sites were generated (bin size = 2). The average of the means (Table 3) is shown by a vertical dotted line. Values corresponding to N0.95, and P0.05 (Table 3) flank the left and right side of the gray region, respectively, and are shown as a dashed/dotted line. The tables within the graphs indicate the percentage of known positive (Pos.) and negative (Neg.) S. purpuratus (Purp.), vertebrate (Vert.), and protostome (Prot.) sequences, which were classified as positive or negative using the average of the means as the threshold.