Figure 2

Comparison of transcript levels between Plk4 heterozygous and wild-type MEFs. A) Confirmation of microarray results. Semi-quantitative RT-PCR was carried out to measure the relative difference in the expression of Plk4 and the candidate genes prohibitin, wisp1 and sap30bp in Plk4 wild-type and heterozygous MEFs. Values in each case were normalized to the levels of GAPDH transcript. Presented data are a result of three independent experiments. B) Relative levels of Plk4 transcript post ionizing radiation (IR). MEFs were exposed to 25 Gy IR and RNA was isolated at the indicated time points post exposure followed by RT-PCR to measure the relative abundance of Plk4 transcript (as above). C) Relative levels of Plk4 transcript post ultraviolet radiation (UV). MEFs were exposed to 40 J/Cm² UV and RNA was isolated at the indicated time points post exposure followed by RT-PCR to measure the relative abundance of Plk4 transcript (as above).