High, clustered, nucleotide diversity in the genome of Anopheles gambiae revealed through pooled-template sequencing: implications for high-throughput genotyping protocols

Table 1 Estimates of nucleotide diversity in mosquitoes (), obtained from different source populations, numbers of loci sequenced (N loci) and sample sizes (N).

Species	Source of sequenced samples	N loci	N		Study
An. gambiae ss	mixed wild population (M)	8	20³	0.0208	[30]
	mixed wild population (M)	14²	20³	0.0043	[30]
	single wild population (M)	109	8	0.0076	[10]
	mixed wild populations (M)	660	10⁴	0.0079	Present study
	3 lab strains (M)	35	7–9	0.0091	[11]
	mixed wild populations (S)	8	22³	0.0191	[30]
	mixed wild populations (S)	14²	22³	0.0043	[30]
	mixed wild population (S)	109	9	0.0092	[10]
	mixed wild populations (S)	660	10⁴	0.0082	Present study
An. arabiensis	single wild population	22	2³	0.0040	[30]
	lab population¹	109	8	0.0064	[10]
An. funestus	single wild population	50	21	0.0079	[31]
Ae. aegypti	3 lab strains	25	n/a	0.0122	[32]

¹recently colonised from field without dramatic bottleneck [10]; ²centromeric X-chromosome loci; ³average N across loci; ⁴single pool of DNA: corrected for elevated Type II error rate.

ISSN: 1471-2164