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Figure 5 | BMC Genomics

Figure 5

From: GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation

Figure 5

A physical map of mouse γ-satellite DNA. A – gel photo of mouse genomic DNA cleavage with restriction enzymes (double digestions). Lanes: 4 – intact DNA; 1, 3, 5, 7, 9 – GlaI added; 1, 2 – AcsI added; 5, 6 – FatI added; 7, 8 – BstF5I added; 9, 10 – Bst2UI added; M – DNA marker pUC19/MspI. B-F – physical maps of mouse γ-satellite DNA with indication of experimentally obtained DNA fragments and theoretically predicted ones. B – GlaI; C – double digestion with GlaI and AcsI; D – double digestion with GlaI and FatI; E – double digestion with GlaI and BstF5I; F – double digestion with GlaI and Bst2UI. Experimental patterns of mouse DNA hydrolysis and DNA ladders are shown on a left side of each figure. Positions of cleavage sites are shown in brackets at the top. Intact GlaI fragments are shown in black, cleaved GlaI fragments are shown in grey, double digestion products are shown in brown. Horizontal dotted lines show a correspondence of predicted fragments (arrows) to those observed on gel photos.

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