Table 1 DNA fragments with mutated or uncleaved ACGT sites
From: GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation
Fragment | Length, bp | Positions of fragment in consensus | Sequence of non-hydrolysed site at position | ||
|---|---|---|---|---|---|
121 | 207 | 61 | |||
G150-5N | 148 | 207 – +121 | - | - | ACGT |
G150-1N | 147 | 207 – +121 | - | - | ACGA |
G150-12N | 148 | 207 – +121 | - | - | ATGT |
G150-29N | 148 | 207 – +121 | - | - | ATGT |
G150-33N | 148 | 207 – +121 | - | - | ACAT |
G234-4 | 234 | 121 – +121 | - | ACGT | TCGT |
G234-8N | 234 | 121 – +121 | - | ACGT | ACTT |
G180-1 | 174 | 121 – +61 | - | ACGT | - |
G180-3 | 174 | 121 – +61 | - | ACGT | - |
G180-6 | 174 | 121 – +61 | - | ACGT | - |
G180-2N | 174 | 121 – +61 | - | ACGT | - |
G180-3N | 173 | 121 – +61 | - | ACGT | - |
G180-4N | 174 | 121 – +61 | - | ACGT | - |
G180-6N | 173 | 121 – +61 | - | ACGT | - |
G234-3 | 234 | 61 – +61 | ACGT | ACGT | - |
G234-13 | 234 | 61 – +61 | ACGT | ACGT | - |
G234-6d | 234 | 61 – +61 | ACAT | ACGT | - |
G234-9N | 234 | 61 – +61 | ACAT | ACGT | - |
G150-4-2 | 146 | 61 – 207 | AGGT | - | - |
G150-6 | 146 | 61 – 207 | TCGT | - | - |
G150-6N | 145 | 61 – 207 | GCAT | - | - |
G150-21N | 146 | 61 – 207 | ACGA | - | - |
G150-22N2 | 146 | 61 – 207 | AAGT | - | - |
G150-24N | 146 | 61 – 207 | ACAT | - | - |
S234-3 | 235 | 98 – +98 | ACAT | CCGT | ACGT |
S234-15N | 233 | 98 – +98 | ACTA | ACGT | ACGT |
A234-3N | 232 | 4 – +4 | ACAT | ACGT | ACGT |
A234-4b | 234 | 4 – +4 | ACAT | ACGT | ACGT |
A234-3 | 233 | 4 – +4 | ACAT | ACGT | ACAT |
S234-13N | 234 | 98 – +98 | ACAT | ACGT | ACAT |
S234-1 | 234 | 98 – +98 | ACGT | ACGT | ACAT |
Number of fragments with non-hydrolyzed ACGT and their percentage | 2/29 | 13/26 | 1/34 | ||
6,9% | 50% | 3,3% | |||
Number of mutated ACGT sites | 14/47 | 1/46 | 9/38 | ||
29,8% | 2,2% | 23,7% | |||