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Table 1 Lower limits of total RNA required for each amplification method.

From: A comparison of RNA amplification techniques at sub-nanogram input concentration

Amplification technique Modified T7 Amplification yield, BT474 RNA(mcg)
140 pg 0.80 – insufficient
280 pg 0.60 – insufficient
375 pg 0.16 – insufficient
500 pg 1.4 – sufficient for 1 array
500 pg 1.7 – sufficient for 1 array
500 pg 0 – insufficient
500 pg 0 – insufficient
500 pg 1.9 – sufficient for 1 array
1 ng 17.8
1 ng 20.3
Arcturus  
100 pg 0 – insufficient
100 pg 0 – insufficient
250 pg 12
250 pg 7.2
500 pg 56.8
1 ng 52.7
Balanced PCR*  
500 pg 0 – insufficient
500 pg 1.5 – sufficient for 1 array
667 pg 2.1 – sufficient for 1 array
667 pg 2.0 – sufficient for 1 array
1 ng 0 – insufficient
1 ng 2.0 – sufficient for 1 array
3.3 ng 2.3 – sufficient for 1 array
3.3 ng 3.2
  1. *1 array discarded due to poor hybridization