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Table 1 Lower limits of total RNA required for each amplification method.

From: A comparison of RNA amplification techniques at sub-nanogram input concentration

Amplification technique Modified T7

Amplification yield, BT474 RNA(mcg)

140 pg

0.80 – insufficient

280 pg

0.60 – insufficient

375 pg

0.16 – insufficient

500 pg

1.4 – sufficient for 1 array

500 pg

1.7 – sufficient for 1 array

500 pg

0 – insufficient

500 pg

0 – insufficient

500 pg

1.9 – sufficient for 1 array

1 ng

17.8

1 ng

20.3

Arcturus

 

100 pg

0 – insufficient

100 pg

0 – insufficient

250 pg

12

250 pg

7.2

500 pg

56.8

1 ng

52.7

Balanced PCR*

 

500 pg

0 – insufficient

500 pg

1.5 – sufficient for 1 array

667 pg

2.1 – sufficient for 1 array

667 pg

2.0 – sufficient for 1 array

1 ng

0 – insufficient

1 ng

2.0 – sufficient for 1 array

3.3 ng

2.3 – sufficient for 1 array

3.3 ng

3.2

  1. *1 array discarded due to poor hybridization