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Table 2 Q-PCR results showing expression changes in 21 genes under cold and salt dehydration conditions plus 18 hours recovery compared to a control sequence.

From: Surviving the cold: molecular analyses of insect cryoprotective dehydration in the Arctic springtail Megaphorura arctica (Tullberg)

  -2 -7 0.9 salt 0.2 salt 18 hr
Aquaporins      
AQP-A (clone ID: sb_006_02P03) -1.50 -3.99 -3.27 +1.89 +1.74
AQP-B (clone ID: sb_006_05H07) -1.67 -2.50 -1.54 -1.67 -1.89
AQP-C (clone ID: sb_006_08O08) +1.05 -1.25 -2.00 -1.12 -1.04
Heat shock proteins      
sHSP +4.65 +5.92 -4.18 -1.35 +8.00
HSP70 +1.06 -2.08 -2.00 +2.00 +1.14
Antioxidants      
Superoxide dismutase -1.62 -2.31 -1.52 -1.50 -1.84
Catalase +3.47 -1.50 -4.55 -2.60 -1.19
Glutathione-S-transferase +37.64 +7.66 -1.50 +3.34 +21.27
Various      
Ferritin +1.87 +2.57 -1.40 +1.19 +1.47
Desaturase +3.42 +5.07 +3.45 -1.50 +6.32
Trehalose GO annotations      
Trehalose 6 phosphate synthase (1) +3.57 +3.50 +1.35 +1.94 +3.68
Trehalose 6 phosphate synthase (2) +6.08 +2.25 +10.10 +15.34 +10.60
LATS tumour supressor -1.15 -2.11 -2.04 -1.30 -1.07
Serine/threonine protein kinase 38 +1.04 -1.17 -1.74 -1.33 +1.61
Protein kinase A cAMP dependant catalytic sub-unit -1.15 -2.15 -2.05 -1.33 -1.07
Trehalase precursor +2.26 +2.28 -2.24 -1.47 +3.35
P70 ribosomal protein S6 kinase -1.14 -1.30 +1.26 -1.10 +2.47
Putative protein kinase DC2 +1.18 -1.08 -1.30 -1.13 +1.35
Similar to serine/threonine protein kinase 6 (Aurora family kinase 1) -3.59 -1.89 -2.68 +1.13 +2.75
Protein kinase C +1.08 -1.51 -2.58 -1.07 +1.43
cAMP dependant protein kinase C1 -3.19 -4.23 -2.01 +1.18 +1.46
  1. The actual change in gene expression is given as calculated via REST [106].