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Table 2 Q-PCR results showing expression changes in 21 genes under cold and salt dehydration conditions plus 18 hours recovery compared to a control sequence.

From: Surviving the cold: molecular analyses of insect cryoprotective dehydration in the Arctic springtail Megaphorura arctica (Tullberg)

 

-2

-7

0.9 salt

0.2 salt

18 hr

Aquaporins

     

AQP-A (clone ID: sb_006_02P03)

-1.50

-3.99

-3.27

+1.89

+1.74

AQP-B (clone ID: sb_006_05H07)

-1.67

-2.50

-1.54

-1.67

-1.89

AQP-C (clone ID: sb_006_08O08)

+1.05

-1.25

-2.00

-1.12

-1.04

Heat shock proteins

     

sHSP

+4.65

+5.92

-4.18

-1.35

+8.00

HSP70

+1.06

-2.08

-2.00

+2.00

+1.14

Antioxidants

     

Superoxide dismutase

-1.62

-2.31

-1.52

-1.50

-1.84

Catalase

+3.47

-1.50

-4.55

-2.60

-1.19

Glutathione-S-transferase

+37.64

+7.66

-1.50

+3.34

+21.27

Various

     

Ferritin

+1.87

+2.57

-1.40

+1.19

+1.47

Desaturase

+3.42

+5.07

+3.45

-1.50

+6.32

Trehalose GO annotations

     

Trehalose 6 phosphate synthase (1)

+3.57

+3.50

+1.35

+1.94

+3.68

Trehalose 6 phosphate synthase (2)

+6.08

+2.25

+10.10

+15.34

+10.60

LATS tumour supressor

-1.15

-2.11

-2.04

-1.30

-1.07

Serine/threonine protein kinase 38

+1.04

-1.17

-1.74

-1.33

+1.61

Protein kinase A cAMP dependant catalytic sub-unit

-1.15

-2.15

-2.05

-1.33

-1.07

Trehalase precursor

+2.26

+2.28

-2.24

-1.47

+3.35

P70 ribosomal protein S6 kinase

-1.14

-1.30

+1.26

-1.10

+2.47

Putative protein kinase DC2

+1.18

-1.08

-1.30

-1.13

+1.35

Similar to serine/threonine protein kinase 6 (Aurora family kinase 1)

-3.59

-1.89

-2.68

+1.13

+2.75

Protein kinase C

+1.08

-1.51

-2.58

-1.07

+1.43

cAMP dependant protein kinase C1

-3.19

-4.23

-2.01

+1.18

+1.46

  1. The actual change in gene expression is given as calculated via REST [106].