Table 1 Sequences (5'-3'), optimal annealing temperatures (°C) and amplified fragment sizes (base pairs) for primers used in the study.
From: Horizontal gene transfer between Wolbachia and the mosquito Aedes aegypti
|  | primer sequence | product size (bp) | optimal annealing temperature (°C) |
|---|---|---|---|
WP1346a* | F-TGGTTGGTCACTACCACGAA R-ACCATTCGGCACTGAAACAT | 432 | 54 |
WP1346b | F-TGAGCATGGTCGTTTATTGG R-CGCACATCTTTCATCCAGAG | 453 | 54 |
WP1346c | F-TCAATCTCGCAAGTTGATGC R-AAATGACCTTGAACGGAAGC | 529 | 54 |
WP1348* | F-ACGACAAGCCTTTTCCTTTG R-TAATATTGCCGGGCTTGTTC | 355 | 54 |
WP1349a* | F-TGGATGTGCGACGTTCTAAG R-TCGGCTGGTAATCCTTTTTG | 399 | 54 |
WP1349b | F-GTGGAATTTTGAAGGCCAAG R-AGGCCCAACATTTTCTTGTG | 372 | 54 |
AAEL004181a* | F-TTCTCCGACCAGATTTTTCC R-AGAAATGTCCCGCCCTTATC | 370 | 54 |
AAEL004181b*^†| F-GAACAAGGGGATCAAGCAAA R-CTTGAATGACCCGAGTGAGA | 475 | 54 |
AAEL004181c*^ | F-CGGAACTCTGGTGGGTACAT R-AGACGTTCGCTTGAAAATCG | 388 | 55 |
AAEL004181d | F-catcggttattgaaccggatac R-caacttcactattctgccaacg | 296 | 54 |
AAEL004181e | F-tcttccgataggttacggattg R-tcgatgtataagcctccatcaac | 251 | 54 |
AAEL004181f*†| F-ACAACCAGTGGAATCCTTCG R-GTTCTCATTTGCGACCCAAT | 318 | 55 |
AAEL004181g†| F-cgctgaaactgtacagacaagg R-tttcattcgtgttgaagtggtc | 273 | 54 |
AAEL004181h†| F-acaccactttcgattgtcattg R-ggcccttcatagctgtagtgac | 260 | 54 |
AAEL004181i*^ | F-GCCATCATCAGGAACCAATC R-CTGATTGCAGCGAGAAATGA | 317 | 55 |
AAEL004188*†| F-TGGACACAAAGACCCATTCA R-AAAACTGGGTGCTTCCATTG | 447 | 53 |