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Table 1 Sequences (5'-3'), optimal annealing temperatures (°C) and amplified fragment sizes (base pairs) for primers used in the study.

From: Horizontal gene transfer between Wolbachia and the mosquito Aedes aegypti

  primer sequence product
size (bp)
optimal annealing temperature (°C)
WP1346a* F-TGGTTGGTCACTACCACGAA
R-ACCATTCGGCACTGAAACAT
432 54
WP1346b F-TGAGCATGGTCGTTTATTGG
R-CGCACATCTTTCATCCAGAG
453 54
WP1346c F-TCAATCTCGCAAGTTGATGC
R-AAATGACCTTGAACGGAAGC
529 54
WP1348* F-ACGACAAGCCTTTTCCTTTG
R-TAATATTGCCGGGCTTGTTC
355 54
WP1349a* F-TGGATGTGCGACGTTCTAAG
R-TCGGCTGGTAATCCTTTTTG
399 54
WP1349b F-GTGGAATTTTGAAGGCCAAG
R-AGGCCCAACATTTTCTTGTG
372 54
AAEL004181a* F-TTCTCCGACCAGATTTTTCC
R-AGAAATGTCCCGCCCTTATC
370 54
AAEL004181b*^ F-GAACAAGGGGATCAAGCAAA
R-CTTGAATGACCCGAGTGAGA
475 54
AAEL004181c*^ F-CGGAACTCTGGTGGGTACAT
R-AGACGTTCGCTTGAAAATCG
388 55
AAEL004181d F-catcggttattgaaccggatac
R-caacttcactattctgccaacg
296 54
AAEL004181e F-tcttccgataggttacggattg
R-tcgatgtataagcctccatcaac
251 54
AAEL004181f* F-ACAACCAGTGGAATCCTTCG
R-GTTCTCATTTGCGACCCAAT
318 55
AAEL004181g F-cgctgaaactgtacagacaagg
R-tttcattcgtgttgaagtggtc
273 54
AAEL004181h F-acaccactttcgattgtcattg
R-ggcccttcatagctgtagtgac
260 54
AAEL004181i*^ F-GCCATCATCAGGAACCAATC
R-CTGATTGCAGCGAGAAATGA
317 55
AAEL004188* F-TGGACACAAAGACCCATTCA
R-AAAACTGGGTGCTTCCATTG
447 53
  1. *denotes primers used for RT-PCR to determine gene expression. ^denotes primers designed to span three introns present in the Aedes aegypti Vectorbase annotation for AAEL004181. †denotes primers used for sequencing Aedes mascarensis PCR products.