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Table 2 Primer pairs used for q-PCR analysis with target gene information.

From: Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations

Primers1

Sequences (5' to 3')

PCR product size (bp)

ZMO0084_F

TGCAAGCATTGCCTACAAAG

100

ZMO0084_R

CCATTGAGGTGAACCCATCT

 

ZMO0178_F

TGATCATCGGTGGTGGTATG

120

ZMO0178_R

TTTCAGCAGCAGCGAAAATA

 

ZMO0367_F

ACAGCCTGATGAAACCATCC

111

ZMO0367_R

AACACATCCGTGAGGGAAAG

 

ZMO0369_F

GCGTTTCTCTATTGCGGAAG

110

ZMO0369_R

CGAAACGTTCCCAAGCTAAC

 

ZMO0749_F

TATCCTGCGGTCTTGGAGTC

108

ZMO0749_R

CCGTCTTCAAAGGCATTCAT

 

ZMO0817_F

GCACCGAAATCAGCAAAAAT

107

ZMO0817_R

GTGTTGGGACTGGGTTCATC

 

ZMO0976_F

CAGCAGATGGACGAGTTCAA

113

ZMO0976_R

TCGTTTTTCTTGGCATAGGG

 

ZMO1062_F

AGGCCAATGACGGTTTACAG

115

ZMO1062_R

GCTTCCTGATCCAAAAGCTG

 

ZMO1118_F

CGCCTGTTATTCTGGTGGAT

109

ZMO1118_R

CGCCTTATTTCAGCCCTATG

 

ZMO1129_F

AGTGAAACCGACTGGCCTAA

104

ZMO1129_R

ATGGTTTCAATCGCAGCTCT

 

ZMO1360_F

TGGTCTCAAGCATCACTTCG

114

ZMO1360_R

CCGCAGTTCAGTTCGTTACA

 

ZMO1478_F

AGTAGCGGTGCTGAACTGGT

109

ZMO1478_R

CGGCAGGCCATTTATAAGAA

 

ZMO1608_F

ACATGGCTAACGACGCTTCT

111

ZMO1608_R

TCTTGATCTGACCGCAGTTG

 

ZMO1660_F

ATGAGCGTCCAGCAATTCTT

102

ZMO1660_R

TTCCCCGACATGACTCAGTA

 

ZMO1863_F

GGAACTCTGGCAGAAACAGC

111

ZMO1863_R

GGATAACCCAAGACGAGCAA

 

ZMO1876_F

ATCAGGATTTGACGCTGGAA

100

ZMO1876_R

ACCATCGCTTCGACAATAGC

 

ZMO1959_F

ACAAGGCTGCCGATTTATTG

104

ZMO1959_R

TTGGCTCAGCAGATGTTGTC

 
  1. 1PCR primer pair nomenclature. F: forward primer, R: reverse primer for each respective gene.
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BMC Genomics

ISSN: 1471-2164

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