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Table 1 Microarray and qRT-PCR comparison

From: Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility

  Microarray Results qRT-PCR Results  
MEF STMN1-/-    
STMN2 -2.44 1.27  
STMN3 N/A N/A  
STMN4 2.26 8.06 *
Tubb2a/Class IIa 1.40 1.80 *
Tubb2b/Class IIb 1.54 2.64 *
Tubb2c/Class IVb -1.35 -0.57 *
Tubb3/Class III 1.22 1.78 *
Tubb6/Class V -1.31 -0.57 *
  1. Magnitude and direction of gene expression changes found using microarray analysis compared to qRT-PCR with designed primers. Only those tubulin isotypes whose expression was significantly changed, as measured by microarray with FDR < 0.05, are shown compared to qRT-PCR (*, p-value < 0.005). Data are given as fold change in STMN-/- MEFs relative to STMN+/+ MEF.