Figure 3

Densities for the evolutionary conservation of 18,717 Z. viviparus transcripts found in at least three of the five available fish genomes. Transcript aligning outside (red line) are significantly more conserved than those aligning inside (blue line) annotated regions (p < 10^-16, Wilcoxon-Mann-Whitney rank sum test). For comparison, a null distribution was generated by calculating the conservation score for random alignments, with similar length as the transcripts aligning outside annotated regions (dashed line).