Figure 1

Distributions of relative start sites and number of reads per gene. A. Start site distributions of 454 sequences for each species in this study including random, oligo-dT, and normalized oligo-dT libraries. Sequencing start sites are calculated as the start position, defined by BLASTn (Arabidopsis) or BLASTx (Eschscholzia, Persea) hit divided by the cDNA or protein length and expressed as percentage of the gene length. B. Distribution of the number of reads from each library mapped to an Arabidopsis gene, defined by best BLASTn or BLASTx hit of each read to the TAIR genes. Species abbreviations are ATH (Arabidopsis thaliana), ECA (Eschscholzia californica), and PAM (Persea americana).