Figure 1From: Global effect of RpoS on gene expression in pathogenic Escherichia coli O157:H7 strain EDL933Growth of EDL933 in LB media. Cultures were inoculated from overnight cultures to a starting OD600 = 0.0001 and incubated aerobically at 37°C at 200 rpm. RNA samples were isolated at OD600 = 0.3 and 1.5 as indicated. RpoS (ðS) protein levels were tested by Western blot analyses using monoclonal anti-RpoS antiserum as described in Materials and Methods. This experiment was performed in triplicate using independent isolates. Averaged values were used for construction of the growth curve.Back to article page