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Table 2 Aromatic degradation in D. aromatica: Mono- and Di-oxygenases.

From: Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation

VIMSS id

Orthologs

Putative function

Size aas

581514

TbuA1/TmoA/TouA/PhlK/Tbc2A

methane/phenol/toluene hydroxylase

501

581515

TbuU/TmoE/TouB/PhlL/Tbc2B

toluene-4-monooxygenase

88

581516

TbuB/TmoC/TouC/PhlM/Tbc2C

ferredoxin subunit of ring-hydroxylating dioxygenase

111

581517

TbuV/TmoD/TouD/PhlN/Tbc2C

monooxygenase

146

581518

TbuA2/TmoB/TouE/PhlO/Tbc2E

hydroxylase

328

581519

TbuC/TmoF/TouF/PhlP/Tbc2F

flavodoxin reductase

338

581520

TbuX/TodX/XylN

membrane protein; transport

464

581521

histidine kinase

signal transduction

963

581522

NarL

cheY like protein

208

581523

methyl-accepting chemotaxis protein

chemotaxis sensory transducer, membrane bound

532

581524

4-oxalocrotonate tautomerase

tautomerase

144

581525

oxidoreductase

oxidoreductase/dehydrogenase

254

581526

MhpE

4-hydroxy-2-oxovalerate aldolase

354

581527

MhpF

EC1.2.1.10 Acetaldehyde dehydrogenase (acetylating)

305

581528

2-hydroxymuconic semialdehyde dehydrogenase

NAD+-dependent dehydrogenase (EC1.2.1.60)

489

581529

ring-cleaving extradiol dioxygenase

catechol 2,3 dioxygenase (1.13.11.2)

311

581530

aldolase

4-hydroxyphenylacetic acid catabolism pathway

266

581531

S box domain

signal transduction

143

584293

orf

unknown

63

581532

orf

unknown

80

584294

EAL domain containing protein (obsolete in current VIMSS database)

diguanylate phosphodiesterase; signaling

65

581533

transcriptional regulator

LysR-type

300

581534

response regulator, tbuT family

activator of aromatic catabolism

558

812947

PhcK/DmpK/PhhK/PheA1/Tcb1A/AphK

monooxygenase

89

581535

PhcL/DmpL/PhhL/PheA2/Tcb1B/AphL

hydroxylase

329

581536

PhcM/DmpM/PhhM/PheA3/Tcb1C/AphM

monooxygenase

89

581537

PhcN/DmpN/PhhN/PheA4/Tcb1D

aromatic hydroxylase

517

581538

PhcO/DmpO/PhhO/PheA5/Tcb1E/AphO

aromatic hydroxylase

118

581539

PhcP/Dmp/PhhP/PheA6/Tcb1F/AphQ

hydroxylase reductase

353

581540

ferredoxin

2Fe-2S ferredoxin, iron-sulfur binding site

112

581541

transcriptional regulator

IPR000524: Bacterial regulatory protein GntR, HTH

235

581542

ring-cleaving extradiol dioxygenase

catechol 2,3 dioxygenase (EC1.13.11.2)

308

581543

orf

unknown

142

581544

2-hydroxymuconic semialdehyde dehydrogenase

NAD+-dependent dehydrogenase (EC1.2.1.60)

484

581545

MhpC

2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase

274

581546/3337834

MhpD

2-keto-4-pentenoate hydratase

260

581547

oxidoreductase

3-oxoacyl-[acyl-carrier-protein] reductase (EC1.1.1.100)

264

581548

MhpF

acetaldehyde dehydrogenase (acetylating; EC1.2.1.10)

304

581549

MhpE

4-hydroxy-2-oxovalerate aldolase

343

581550

hydratase/decarboxylase

4-oxalocrotonate decarboxylase

262

581551

tautomerase

4-oxalocrotonate tautomerase

63

  1. The large cluster of aromatic degradation enzymes in the D. aromatica genome shown includes two mono-oxygenase clusters in a linear array on the D. aromatica chromosome, with 17 predicted genes intergenically inserted, which encode mhp 'cluster 6' and several predicted signaling proteins. The second monooxygenase cluster is followed by mhp 'cluster 2' (for an overview of mhp clusters see Fig. 1).