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Table 5 Hydrogenase clusters associated with nitrogen fixation.

From: Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation

VIMSS id

Orthologs

Putative function

Size, aas

581358

HoxK/HyaA/HupS

hydrogenase-1 small subunit

363

581359

HoxG/HyaB/HupL

hydrogenase-1, nickel-dependent, large subunit

598

581360

HoxZ/HyaC/HupC

Ni/Fe-hydrogenase 1 b-type cytochrome subunit

234

581361

HoxM/HyaD/HupD

hydrogenase expression/formation protein

204

581362

HoxL/HypC/HupF

hydrogenase assembly chaperone

100

581363

HoxO/HyaE/HupG

hydrogenase-1 expression

152

581364

HoxQ/HyaF/HupH

nickel incorporation into hydrogenase-1 proteins

287

581365

HoxR/HupI

rubredoxin-type Fe(Cys)4 protein

66

581366

HupJ/(similar to HoxT)

hydrogenase accessory protein

156

581367

HoxV/HupV

membrane-bound hydrogenase accessory protein

308

581368

HypA

hydrogenase nickel insertion protein

113

581369

HypB

hydrogenase accessory factor Ni(2+)-binding GTPase

352

581370

HypF

hydrogenase maturation protein

763

581371

ABC protein

periplasmic component, ABC transporter

260

581372

GGDEF domain

signal transduction, GGDEF

523

581373

Hyb0

hydrogenase-2 small subunit

394

581374

HybA

Fe-S-cluster-containing hydrogenase component

351

581375

HybB

cytochrome Ni/Fe component of hydrogenase-2

386

581376

HybC/HynA

hydrogenase-2 large subunit

570

581377

HybD/HynC

Ni, Fe-hydrogenase maturation factor

159

581378

HupF/HypC

hydrogenase assembly chaperone

96

581379

HybE/HupJ

hydrogenase accessory protein

183

581380

HypC

hydrogenase maturation protein

81

581381

HypD

hydrogenase maturation protein

374

581382/3337851

HypE

hydrogenase maturation protein

330

581383

HoxX/HypX

formation of active hydrogenase

558

581384

HoxA

response regulator with CheY domain (signal transduction)

495

581385

HoxB/HupU

regulatory [NiFe] Hydrogenase small subunit (sensor)

333

581386

HoxC/HupV

regulatory [NiFe] Hydrogenase large subunit (sensor)

472

581397

HupT

histidine kinase with PAS domain sensor

448

581398

HoxN/HupN/NixA

nickel transporter

269

  1. A large region of putative hydrogenases associated with nitrogen fixation is noted. Hydrogenase-1 is composed of hox/hup genes, hydrogenase-2 of the hyb genes, and the nickel insertion/maturation complex, hyp (present in two clusters: hyp ABF and hyp CDE).