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Table 3 Melting temperature (T m ) of G-quadruplex sequences from the upstream B regions of var genes and the equilibrium dissociation constant of the PQS with the G-quadruplex ligand BOQ 1 (shown in the Kd column)

From: Putative DNA G-quadruplex formation within the promoters of Plasmodium falciparum var genes

 

T m 1

 

PQS

K+

Na+

NH4+

Kd (μM)

UpsB-Q-1

47.2 ± 0.6

35.1 ± 1.2

31.6 ± 0.7

2.6 ± 0.5

UpsB-Q-2

49 ± 0.5

36.1 ± 2

27.7 ± 0.5

1.4 ± 0.4

UpsB-Q-3

50 ± 1.2

36.9 ± 1.1

34.2 ± 1.1

1.7 ± 0.7

UpsB-Q-4

49.3 ± 1.4

39.4 ± 0.9

32.3 ± 0.3

2.7 ± 1.1

  1. 1 Thermal denaturation experiments were performed at the cation concentration of 150 mM. Thermal denaturation experiments were repeated twice and T m values shown are the mean of four values obtained from four curves (two heatings and two coolings).
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BMC Genomics

ISSN: 1471-2164

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