Maps of expressed and donor MSG genes. The expressed MSG gene is adjacent to the UCS sequence. Donor MSG genes are not adjacent to the UCS sequence, which is unique in the genome. For illustrative purposes, just three of approximately 80 donor genes are depicted. There is a copy of the CRJE, which is a 24 basepair conserved sequence, at the beginning of MSG genes, including the one attached to the UCS. The discontinuities in the MSG genes are there to indicate that the genes are not drawn to size relative to the UCS and CRJE. The open horizontal arrows show the locations and orientations of PCR primers. The two arrows above the expressed gene represent the -145 and α-CRJE primers (Additional file 3, Table S2). The two arrows above the first donor gene represent the CRJE and C2 primers (Additional file 3, Table S2).