Figure 4

RT-PCR amplifications. RT-PCR amplifications of some transcripts differentially expressed or control (ie, not differential) between the 12 or 10 zones studied. T: Testis, VE: Vas efferens, PC: Proximal caput (E0-1), MC: Median caput (E2), DC: Distal caput (E3-4), Corpus (E4-7), Cauda (E8/9 and Vas deferens (VD)), (-): negative PCR controls. The table on right indicated the number of PCR cycles used and the cluster in which the transcript belongs by the clustering analysis using 12 or 10 zones. NC: transcripts were not classified in an identical manner by the two methods (PAM and HCL), NA: transcripts were not statistically differentially expressed. INSL3 (insulin-like 3), INHA (inhibin, alpha), CLU (clusterin), METTL7B (methyltransferase like 7B), RARRES2 (retinoic acid receptor responder (tazarotene induced) 2), TEDDM1 (Transmembrane epididymal protein 1), SPP1 (secreted phosphoprotein 1), TSPAN1 (tetraspanin 1), GPX5 (glutathione peroxidase 5 (epididymal androgen-related protein)), HEXA (hexosaminidase A (alpha polypeptide)), RAP1A (RAP1A, member of RAS oncogene family), CPE (carboxypeptidase E), CES7 (carboxylesterase 7), MAN2B2 (mannosidase, alpha, class 2B, member 2), MUC1 (mucin 1, cell surface associated), AWN (sperm associated AWN protein), CRABP1 (cellular retinoic acid binding protein 1), RERG (RAS-like, estrogen-regulated, growth inhibitor), APLP2 (amyloid beta (A4) precursor-like protein 2), B2M (beta-2-microglobulin).