Figure 8

Protease inhibitors expression. A to D: Relative expression of WFDC3 (WAP four-disulfide core domain 3), SLPI (secretory leucocyte peptidase inhibitor), WFDC2 (WAP four-disulfide core domain 2), UNQWAP (uncharacterised WAP four-disulfide core domain protein) is represented as the percent of total intensity measured in the 12 samples studied by microarray in black and by RT-PCR values (grey). T: Testis, VE: Vas efferens, 0 to 8/9: nine epididymal zones, VD: Vas deferens). On the top the image of the agarose gel used for quantification. E to H: Comparison of the amino acid sequences of deduced Whey Acidic Protein family from different mammalian species. Identical Amino acid residues between at least two sequences in alignments are placed in shaded boxes. Characteristic domains are boxed and residues of the WAP domain are in bold. Hyphens represent gaps introduced for optimal alignment. Accession number of sequences used for alignments are: E: Mus (NP_082237), Homo (CAC36106), Sus (unpublished), Bos (XP_001789331); F: Sus_A (translated from scac0031.l.24), Sus_B (P22298), Mus (NP_035544), Homo (NP_003055), Bos (XP_590087); G: Sus (NP_999112), Homo(NP_006094), Bos (NP_001069958), Mus (NP_080599) and H: Mus (NP_001012741), Sus (translated from BX915529), Homo (NP_542791).