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Table 5 Cost per sample and number of mapped reads with increasing multiplexing

From: Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing

Number of barcoded adapters

Fraction of non-barcoded cost per sample*

Number of mapped reads**

1

100%

10.00 M

4

34.61%

2.50 M

8

23.71%

1.25 M

12

20.08%

0.83 M

16

18.26%

0.63 M

40

15.00%

0.25 M

  1. *Includes Illumina DNA library preparation (fixed cost), sequencing run
  2. *Excludes ChIP reagents, Illumina GAII sequencer, HR and data storage
  3. *For a non-barcoded sample, the cost per sample is $498.23 USD as of October 2008.
  4. **Assumes optimal mapping values for a lane run on a GA II (10 million mapped reads)
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BMC Genomics

ISSN: 1471-2164

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