Heat map of genes significantly regulated during the life-cycle of Trypanosoma cruzi. Ratios are log2 (stage/reference), thus yellow bars represent upregulation and blue bars represent downregulation. Two trypomastigote upregulated clusters are expanded. The spots annotated as 'not mappable to annotated ORF' were designed from earlier versions of the T. cruzi genome sequence and subsequently designated as 'obsolete' by TIGR. These spots no longer mapped within an annotated ORF in the final version of the genome sequence, e.g. they were in intergenic regions (such as untranslated regions) and/or were antisense (would hybridize with first strand cDNA from transcripts from the non-coding strand).