Duplicate genes in Trypanosoma cruzi show divergent expression patterns. Microarray expression data for paralog groups containing 3–15 members were compared to identify groups showing divergent expression between duplicate genes. Shown are selected groups with representative divergent expression patterns. Gene ID's are official gene symbols (prefix Tc00.1047053 is truncated) assigned to annotated genes in the reference CL Brener T. cruzi genome. Expression data for the amastins were confirmed by qRT-PCR (top right panel). Error bars are standard deviations for three biological replicates for both the microarray and qRT-PCR data. The qRT-PCR and microarray standard deviations reflect biological, not technical variation.