Figure 5

Kcnj9 B6 vs. D2 PARC SNPs. Kcnj9 is represented with the coding region shown in the gray boxes and the 5' and 3' untranslated regions (UTRs) in the white boxes. Introns are illustrated as V-lines, and upstream region as a horizontal black line. The blue lines indicate SNPs found in MPD that we confirmed by custom HTS (PARC SNPs). The red lines indicate previously cryptic SNPs, i.e., new PARC SNPs. For Kcnj9 (ENSMUST00000062387), we found nine new SNPs in the 3'-UTR, and 18 new SNPs in the 2 kb upstream region, and identified one false positive SNP (green line) in the 3'-UTR. In addition, we confirmed six synonymous coding SNPs, eleven 3'-UTR SNPs, one 5'-UTR SNP, and 5 SNPs within 2 kb upstream of the transcription start site. Additionally, within the introns, we identified 15 new SNPs and confirmed 28 SNPs (not shown).