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Figure 4 | BMC Genomics

Figure 4

From: The degree of microbiome complexity influences the epithelial response to infection

Figure 4

Accelerated HIGK proliferation upon infection with P. gingivalis is inhibited in co-culture with S. gordonii. A) Total interaction and invasion levels determined by measuring the total numbers of P. gingivalis associated with HIGK cells by live counts in mono-(Pg) and in mixed infection (Mixed) with S. gordonii at the highest MOI. Results are the mean of three experiments. B) HIGK cells at a low confluency were co-cultured with single and complex mixtures of bacteria and cultured for up to 144 hours. Labels: Control, uninfected HIGK; Sg, S. gordonii; Pg, P. gingivalis; MIXED, co-infection with S. gordonii and P. gingivalis at different multiplicity of infection (MOI). All cell counts were performed in triplicate and all experiments were repeated twice. * p > 0.05 P. gingivalis vs non-infected controls; #p < 0.05 S. gordonii vs. non-infected controls; ^p < 0.05 S. gordonii co-infection with P. gingivalis (2500:1) vs. non-infected controls (p < 0.05) by ANOVA with Dunnett's Multiple Comparison Test.

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