Effect of CAR activation on selected metabolic pathways in normal and hyperlipidemic mouse livers

BMC Genomics

Table 3 Expression of genes in wild type mice treated with TCPOBOP as detected by RT-PCR

Treatment	TCPOBOP	Untreated	TCPOBOP
Diet	Standard diet	1%cholesterol vs standard diet	1%cholesterol diet
Hmgcr	3.09 ± 2.93*	0.27 ± 0.14*	2.42 ± 0.76*
Cyp51a1	1.80 ± 1.49	0.34 ± 0.24*	3.00 ± 1.33*
Srebp2	1.18 ± 0.56	0.41 ± 0.11*	1.58 ± 0.47*
Insig1	2.66 ± 2.19*	0.96 ± 0.42	1.36 ± 0.53
Insig2a	8.34 ± 5.54*	1.43 ± 0.32*	2.78 ± 1.12*
Insig2b	0.24 ± 0.11*	0.74 ± 0.20	0.37 ± 0.20*
Cyp7a1	1.29 ± 1.74	0.85 ± 0.44	0.81 ± 0.64
Cyp8b1	0.34 ± 0.41*	0.69 ± 0.29	0.43 ± 0.22*
Cyp2b10	291.4 ± 194.5*	1.04 ± 0.31	135.4 ± 40.5*
Cyp3a11	11.17 ± 6.13*	/	10.8 ± 4.98*

The expression of key genes involved in cholesterol homeostasis and drug metabolism as measured by RT-PCR. Data represents fold change ± standard deviation of TCPOBOP treated versus vehicle treated on standard diet, cholesterol diet, and vehicle treated group on cholesterol diet versus standard diet. Six animals per group were analyzed and statistical significance was determined using Student t-test. Unigene symbols are used as abbreviations. *p < 0.05.

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