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Table 6 Expression of genes in knock-out mice treated with TCPOBOP as detected by RT-PCR

From: Effect of CAR activation on selected metabolic pathways in normal and hyperlipidemic mouse livers

Treatment

Gene name

Genotype

  

WT

CAR -/-

TCPOBOP

Hmgcr

2.22 ± 1.38

0.35 ± 0.18*

 

Sqle

2.18 ± 1.1*

0.69 ± 0.25*

 

Cyp51a1

1.43 ± 0.89

0.43 ± 0.16*

 

Insig1

1.86 ± 0.77

0.55 ± 0.24*

 

Srebp2

1.31 ± 0.73

0.49 ± 0.20*

 

Cyp3a11

5.82 ± 3.35*

0.44 ± 0.36

 

Cyp2b10

5750 ± 2805*

3.59 ± 5.61

Untreated CAR -/- vs WT

Hmgcr

-

4.1 ± 1.26*

 

Sqle

-

2.90 ± 0.55*

 

Cyp51a1

-

2.81 ± 0.74*

 

Insig1

-

3.38 ± 1.02*

 

Srebp2

-

3.95 ± 1.14*

 

Cyp3a11

-

3.03 ± 1.80

 

Cyp2b10

-

0.76 ± 0.54

  1. The effect of TCPOBOP and CAR on the expression of cholesterol synthesis and Srebp2 signaling genes as measured by RT-PCR. Cyp3a11 and Cyp2b10 serve as positive controls for CAR activated genes. Data represents fold changes ± standard deviation of treated versus vehicle treated or untreated CAR -/- versus wild type (WT). All animals were fed standard chow diet. Six animals per group were analyzed and statistical significance was determined using Student t-test. Unigene symbols were used as abbreviations. *p < 0.05.
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BMC Genomics

ISSN: 1471-2164

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