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Table 3 Q-PCR expression data for relevant validated genes

From: Extending the models for iron and sulfur oxidation in the extreme Acidophile Acidithiobacillus ferrooxidans

ID NC011761 Gene (locus) Function log2 (Fe/S) Induced Proteomic data: strain/references
rus operon
AFE_3146 rus rusticyanin 3,8 Fe ATCC 33020/[7] CCM 4253/[24] ATCC 19859/[25]
AFE_3151 cup conserved hypothetical protein 4,9 Fe ATCC 33020/[7]
Cytochrome c oxidase complex biogenesis operon
AFE_3141 ctaR iron responsive regulator of the Rrf2 family 3,9 Fe  
Sensor/regulator two-component signal transduction system
AFE_3137 regA DNA-binding response regulator 4,3 Fe  
petI operon
AFE_3108 sdrA1 oxidoreductase, short-chain dehydrogenase/reductase family 3,7 Fe  
AFE_3109 petA1 ubiquinol-cytochrome c reductase, iron-sulfur subunit 6,4 Fe CCM 4252/[24]
AFE_3110 petB1 ubiquinol-cytochrome c reductase, cytochrome b subunit 5,7 Fe  
AFE_3111 petC1 ubiquinol-cytochrome c reductase, cytochrome c 1 subunit 5,5 Fe  
Others
AFE_2599 -   1,2 Fe  
AFE_3116 -   1,2 Fe  
AFE_3119 -   3,4 Fe  
AFE_3124 cysD sulfate adenylyltransferase, small subunit 4,8 Fe CCM 4253/[24]
Thiosulfate-quinone oxidoreductase complex operon
AFE_0043 - periplasmic solute-binding protein, putative -2,5 S CCM 4253/[24] ATCC 19859/[25] ATCC 23270/[29]
AFE_0045 - sulfur/pyrite/thiosulfate/sulfide-induced protein -1,2 S ATCC 19859/[25] ATCC 19859/[28]
Cytochrome bd ubiquinol oxidase
AFE_0955 cydA cytochrome d ubiquinol oxidase, subunit I -2,0 S  
Cytochrome bo 3 ubiquinol oxidase      
AFE_0632 cyoB cytochrome o ubiquinol oxidase, subunit I -3,2 S  
Heterodisulfide reductase complex operon
AFE_2553 hdrA pyridine nucleotide-disulfide oxidoreductase -1,3 S  
AFE_2555 hdrC iron-sulfur cluster-binding protein -1,6 S  
AFE_2586 hdrB heterodisulfide reductase subunit B, homolog -0,8 S  
Sulfide-quinone reductase
AFE_1792 sqr Sulfide-quinone reductase -0,1 CCM 4253/[24] NASF-1/[26]
Others
AFE_0049 - periplasmic solute-binding protein, putative 0,3 ATCC 23270/[29]
AFE_1663 glcF glycolate oxidase, iron-sulfur subunit -1,7 S  
AFE_1677 cbbOIa von Willebrand factor type A domain protein -1,9 S  
AFE_2971 cysN2 sulfate adenylyltransferase, large subunit -1,3 S  
AFE_0282 fur ferric uptake regulator 0,6  
AFE_2324 pgm phosphoglucomutase 0,6  
AFE_0445 galU UTP-glucose-1-phosphate uridylyltransferase 0,2  
AFE_1342 epsS UDP-glucose 4-epimerase 0,0  
AFE_2840 malQ glycosyl hydrolase -0,2  
AFE_2836 glbB 1,4-alpha-glucan branching enzyme -0,6  
AFE_3054 cbbOIb von Willebrand factor type A domain protein -0,1  
AFE_2157 cbbOII von Willebrand factor type A domain protein -0,6  
AFE_0539 cysN3 sulfate adenylyltransferase, large subunit 0,1  
AFE_2602 - hypothetical -0,1 ATCC 19859/[25]
  1. Real time PCR gene expression values (log2 ratio of median) for relevant genes alluded in the revised iron or sulfur oxidation models for A. ferrooxidans ATCC 23270. Genes with a log2 ratio of median larger than |1.5| (corresponding to genes induced more than 2.8 fold) are considered differentially expressed (indicated with *). Gene ID is that of Genbank genome annotation NC 011761. The reference and the strain in which the level of the gene product has been shown to be higher in one of the conditions tested (Fe(II) or S0) are indicated in the last column.