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Table 3 Q-PCR expression data for relevant validated genes

From: Extending the models for iron and sulfur oxidation in the extreme Acidophile Acidithiobacillus ferrooxidans

ID NC011761

Gene

(locus)

Function

log2 (Fe/S)

Induced

Proteomic data: strain/references

rus operon

AFE_3146

rus

rusticyanin

3,8

Fe

ATCC 33020/[7]

CCM 4253/[24]

ATCC 19859/[25]

AFE_3151

cup

conserved hypothetical protein

4,9

Fe

ATCC 33020/[7]

Cytochrome c oxidase complex biogenesis operon

AFE_3141

ctaR

iron responsive regulator of the Rrf2 family

3,9

Fe

 

Sensor/regulator two-component signal transduction system

AFE_3137

regA

DNA-binding response regulator

4,3

Fe

 

petI operon

AFE_3108

sdrA1

oxidoreductase, short-chain dehydrogenase/reductase family

3,7

Fe

 

AFE_3109

petA1

ubiquinol-cytochrome c reductase, iron-sulfur subunit

6,4

Fe

CCM 4252/[24]

AFE_3110

petB1

ubiquinol-cytochrome c reductase, cytochrome b subunit

5,7

Fe

 

AFE_3111

petC1

ubiquinol-cytochrome c reductase, cytochrome c 1 subunit

5,5

Fe

 

Others

AFE_2599

-

 

1,2

Fe

 

AFE_3116

-

 

1,2

Fe

 

AFE_3119

-

 

3,4

Fe

 

AFE_3124

cysD

sulfate adenylyltransferase, small subunit

4,8

Fe

CCM 4253/[24]

Thiosulfate-quinone oxidoreductase complex operon

AFE_0043

-

periplasmic solute-binding protein, putative

-2,5

S

CCM 4253/[24]

ATCC 19859/[25]

ATCC 23270/[29]

AFE_0045

-

sulfur/pyrite/thiosulfate/sulfide-induced protein

-1,2

S

ATCC 19859/[25]

ATCC 19859/[28]

Cytochrome bd ubiquinol oxidase

AFE_0955

cydA

cytochrome d ubiquinol oxidase, subunit I

-2,0

S

 

Cytochrome bo 3 ubiquinol oxidase

     

AFE_0632

cyoB

cytochrome o ubiquinol oxidase, subunit I

-3,2

S

 

Heterodisulfide reductase complex operon

AFE_2553

hdrA

pyridine nucleotide-disulfide oxidoreductase

-1,3

S

 

AFE_2555

hdrC

iron-sulfur cluster-binding protein

-1,6

S

 

AFE_2586

hdrB

heterodisulfide reductase subunit B, homolog

-0,8

S

 

Sulfide-quinone reductase

AFE_1792

sqr

Sulfide-quinone reductase

-0,1

≈

CCM 4253/[24]

NASF-1/[26]

Others

AFE_0049

-

periplasmic solute-binding protein, putative

0,3

≈

ATCC 23270/[29]

AFE_1663

glcF

glycolate oxidase, iron-sulfur subunit

-1,7

S

 

AFE_1677

cbbOIa

von Willebrand factor type A domain protein

-1,9

S

 

AFE_2971

cysN2

sulfate adenylyltransferase, large subunit

-1,3

S

 

AFE_0282

fur

ferric uptake regulator

0,6

≈

 

AFE_2324

pgm

phosphoglucomutase

0,6

≈

 

AFE_0445

galU

UTP-glucose-1-phosphate uridylyltransferase

0,2

≈

 

AFE_1342

epsS

UDP-glucose 4-epimerase

0,0

≈

 

AFE_2840

malQ

glycosyl hydrolase

-0,2

≈

 

AFE_2836

glbB

1,4-alpha-glucan branching enzyme

-0,6

≈

 

AFE_3054

cbbOIb

von Willebrand factor type A domain protein

-0,1

≈

 

AFE_2157

cbbOII

von Willebrand factor type A domain protein

-0,6

≈

 

AFE_0539

cysN3

sulfate adenylyltransferase, large subunit

0,1

≈

 

AFE_2602

-

hypothetical

-0,1

≈

ATCC 19859/[25]

  1. Real time PCR gene expression values (log2 ratio of median) for relevant genes alluded in the revised iron or sulfur oxidation models for A. ferrooxidans ATCC 23270. Genes with a log2 ratio of median larger than |1.5| (corresponding to genes induced more than 2.8 fold) are considered differentially expressed (indicated with *). Gene ID is that of Genbank genome annotation NC 011761. The reference and the strain in which the level of the gene product has been shown to be higher in one of the conditions tested (Fe(II) or S0) are indicated in the last column.