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Figure 1 | BMC Genomics

Figure 1

From: Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis

Figure 1

Experimental design and reproducibility of qPCR-array. A, The same RNA (500 ng) was used in two different reverse transcription (RT) reactions and the products were used in qPCR-arrays performed on different days. B and C, inter-assay reproducibility as determined by comparison of raw Ct values between replicates of different day reactions. D and E, inter-sample reproducibility as determined by comparison of raw Ct values between replicates of different RT reactions. Corresponding r and p values were determined by linear regression analysis. Sample number presented in each plot.

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