FISH to mitotic metaphase chromosomes of T. aestivum. The cultivars analyzed are (a, b) T. aestivum cv. Chinese Spring and (c, d) T. aestivum cv. Renan. Probes were labeled with biotin and digoxygenin, detected with avidin-FITC (green) and anti-digoxygenin-rhodamine (red), respectively. The yellow signal arises from colocalized probes. The probe combinations: (a) BAC_2322J20 (green) and pSc119 (red); (b) BAC fragment (2322J20/30) (green) and pSc119 (red); (c) BAC_2050O8 (green) and pSc119 (red); and (d) Spelt52_2050O8 (red) and pSc119 (green).