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Table 1 Tol2 remobilization screen overview

From: Genome-wide analysis of Tol2 transposon reintegration in zebrafish

Screen

1sta

2ndb

3rdc

Total F0 fish screened

282

268

175

Total F0 founder fishd

21

103

84

Apparent germline transposition ratee

7%

38%

48%

Number of new insertionsf

23

172

143

  1. a The SqET33 line was used as a donor and mRNA containing only an ORF for the transposase was injected into embryos. b The SqET33 line was used as a donor and transposase mRNA containing the 5'- and 3'-UTRs of the Xenopus β-globin gene was injected into embryos. c The SqET33-E20 line, a derivative of SqET33, was used as a donor and transposase mRNA containing the 5'- and 3'-UTRs of the Xenopus β-globin gene was injected into embryos. d Fish with transposon reintegration into germline. The progeny (F1) of these fish showed new GFP expression patterns different from the original donor one. e Rates are calculated as the percentage of F0 founder fish. Since we counted only F0 fish producing progeny with new GFP expression patterns, these rates are likely to be underestimates. f Some F0 founder fish have more than one insertion in the germline identified by TAIL-PCR.