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Table 2 Number of Tol2 insertions per F0 germline

From: Genome-wide analysis of Tol2 transposon reintegration in zebrafish

Number of insertions/F0 germlinea

Number of F0 founder fish (n = 208)

1

122 (59%)

2

48 (23%)

3

27 (13%)

4

8 (4%)

5

2 (1%)

6

0

7

1 (0.5%)

Donor insertionb

36 (17%)

  1. a F0 founder fish were outcrossed to wild type fish and the resulting embryos showing new GFP expression patterns were analyzed by TAIL-PCR. b The donor insertion retained after reintegration was confirmed by TAIL-PCR. These are cumulative data from three rounds of screening.