Reciprocal regulation of microRNA and mRNA profiles in neuronal development and synapse formation

BMC Genomics

Table 1 miRNA targeting of neural progenitor maintenance genes

	Target Gene
miRNA ID	Fgf2	Fgfr1	Efnb1	Rgs3	Notch1	Mef2d
mmu-let-7 family						(2..8) CUACCUC
mmu-miR-15b and 16	(1..7) GCUGCUA × 2	(2..8) UGCUGCU	(1..7) GCUGCUA
mmu-miR-17 and 20a			(2..8) GCACUUU
mmu-miR-25				(2..8) GUGCAAU	(2..7) UGCAAU	(2..8) GUGCAAU
mmu-miR-30c					(1..7) GUUUACA
mmu-miR-103	(1..8) AUGCUGCU		(2..7) UGCUGC			(2..8) AUGCUGC
mmu-miR-124			(2..8) GUGCCUU
mmu-miR-125 family						(1..7) UCAGGGA

Evolutionarily conserved sites complementary to seed regions of highly expressed miRNAs (among top 30 most expressed) were found in 3'UTRs of the genes previously shown to be involved in maintenance of neural progenitors: Fgf2 - fibroblast growth factor 2 [35], Fgfr1 - fibroblast growth factor receptor 1 [35], Efnb1 - ephrin B1 [36], Rgs3 - PDZ-RGS3 [36], Notch1 [34] and Mef2d - myocyte enhancer factor 2D [70]. RNA sequences in uppercase represent the seed matching sites (from TargetScan [21]) of aligned 3'UTRs. All sequences are conserved between human, mouse, rat and dog. Numbers in parentheses show the the positions of mature miRNA sequences to which the 3'UTR sites are complementary.

ISSN: 1471-2164