Figure 4

Verification of ASE assays using Solexa. Each data point represents one of three replicate dilutions analyzed at each step of the dilution series (9:1, 8:2, 7:3, 5:5, 3:7, 2:8, 1:9). The distribution of sequencing reads within each gene is demonstrated as follows: mean ± SE (n = 21). DSX = 11.3 ± 3.8, CG2604 = 2,478.3 ± 288.8, CG10824 = 2,756.4 ± 333.5, CG11459 = 11,578.6 ± 2515.1.