Table 4 Forward and reverse primers sequences for signature and normalization genes.
Gene Name | Accession number | Primer sequence |
|---|---|---|
4-genes signature | ||
myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2) | NM_002466 | -AGCAAGTGCAAGGTCAAATGG -CTGTCCAAACTGCCTCACCA |
karyopherin alpha 2 (KPNA2) | NM_002266 | -TAAGGCAGATTTTAAGACACAAAAGG -GTTCAACTGTTCCACCACTGGTATA |
cell division cycle 2 (CDC2) | NM_001786 | -GCCGCCGCGGAATAAT -CCTTCTCCAATTTTCTCTATTTTGGT |
cell division cycle 20 homolog (CDC20) | NM_001255 | -CTTCCCTGCCAGACCGTATC -CCAATCCACAAGGTTCAGGTAATA |
Genes of Normalization | ||
glucuronidase beta (GUS) | NM_000181 | -GAGTGGTGCTGAGGATTGGC -TCTAGCGTGTCGACCCCATT |
TATA box binding protein (TBP) | NM_003194 | -GCCCGAAACGCCGAATAT -TCGTGGCTCTCTTATCCTCATGA |
ribosomal protein, large, P0 (RPLP0) | NM_001002 | -ACCAAGGAGGACCTCACTGAG -ACCAGCACGGGCAGCAG |
transferrin receptor (TFRC) | NM_003234 | -GGAGCCAGGAGAGGACTTCC -TTCTCCGACAACTTTCTCTTCAGG |
ER | ||
estrogen receptor 1 (ESR1) | NM_000125 | -TGTTCCAAACCCATCGTCAGT -GCACCTGCTCATGGGACAA |